Agilent 1100 Calibration Table

[munkietoes]

Does anyone have a preferred method of calculating calibration tables using the Agilent 1100 HPLC? I currently use a bracketing method but unfortunately you can't do a partial sequence, if for example you need to rerun one of the standards. Does anyone either know how to get around this or if there is a better way of generatinga calibration table using the software? Thanks in advance!

[Tim]

Which software are you using to control the 1100s?

[munkietoes]

ChemStation Rev. B.01.03

[Tim]

Unfortunately not a version I have used, though I believe your version has similarities with Cerity, which we have, so I'll base my reply on that. I've also used older versions on ChemStation (A.09 and below).

Since you can't run partial sequences, you've got a couple of options. Either you'll have to create a one off small sequence for reinjection, including standards with that, or you could (if your versino of ChemStation will do it), use a method with Method specific calibration, as opposed to sequence specific calibration. That way you could just do the reinjection sequence without any standards and it would use the calibration data from the method (and hence previous sequence run).

In Cerity we gave up using the built in calibration and instead do everything through the custom calculator. We use an overal mean standard area in calculations from that, which is therefore unique to a sequence.

Sorry I'm not of much help in your case, but this may give some pointers.

[Bruce Hamilton]

Sorry don't have the software on an 1100, but I wonder if you could try using the cyclic recalibration with bracketing for a partial sequence?.

Bruce Hamilton

[munkietoes]

I will try your suggestions and hopefully with some luck I can give you an update on what worked! Thanks so much!

[Jayesh Patel]

Hi
Can you tell me, what the problem if you are using partial sequence.....?

[munkietoes]

The problem I was having was that I was using a bracketed calibration method. This method unfortunately doesn't allow for a partial sequence. Such as reinjecting a sample if you realized you preped it incorrectly, as I had done.