Question about Shimadzu LCMS-8040 Optimization tool for MRM
Posted: Sat Oct 22, 2016 10:45 pm
Hi all.
I´m working with a Shimadzu LCMS-8040 and I was doing a sensitivity test with reserpine. In the S/N test we inject a 1ng/uL of reserpine.
I set the precursor and the product ion as indicated in the protocol, 609,3>195.0. The signal result was about S:1500 and the S/N:1100.
Then used the optimization tool and the precursor ion was adjusted to 609,8>195.0, run the test but the signal decreases a lot. S:150.
I set the precursor ion back to 609,3 again and run the test. The result was S:2572 and the S/N:1287.
I would like to know if someone has seen this, if it is a particular case with this molecule or if it is some resolution of mass issue due to the tuning file.
I´m working with a Shimadzu LCMS-8040 and I was doing a sensitivity test with reserpine. In the S/N test we inject a 1ng/uL of reserpine.
I set the precursor and the product ion as indicated in the protocol, 609,3>195.0. The signal result was about S:1500 and the S/N:1100.
Then used the optimization tool and the precursor ion was adjusted to 609,8>195.0, run the test but the signal decreases a lot. S:150.
I set the precursor ion back to 609,3 again and run the test. The result was S:2572 and the S/N:1287.
I would like to know if someone has seen this, if it is a particular case with this molecule or if it is some resolution of mass issue due to the tuning file.