can someone help me find a system for this asap?

[imonic]

Hey, I'm having a really hard time determining what system to use in order to detect cetrimide (alkyltrimethylammoniumbromide). Can anyone please help me? So far I've tried mixtures of methanol, acetonitrile and toluenesulphonic, but I can't seem to find it. Please Help me, thank you very much.

[SIELC_Tech]

Your problem is in detection technique (I am assuming that you are doing HPLC). Your compound is not UV active. You need ELSD or LC/MS detector to monitor cetrimide. You can also use indirect detection.
Any reverse column will retain cetrimide. You can use ACN-water-TFA or ACN-water-ammonium formate for ELSD/LC-MS. 50-70% ACN should give you elution

[Chris Pohl]

imonic,

A good column to use for analytes of this sort is the Acclaim Surfactant column from Dionex. If you follow this link you'll find a description as well as a data sheet on the column which includes example chromatograms at least one of which includes your analyte of interest: http://www1.dionex.com/en-us/columns_ac ... 25924.html