Sequence % area in Agilent Cerity for pharm QA/QC.

[Bruce Hamilton]

Hi folks,

I have a standalone Agilent Cerity v2.02 on an 1100 LC, having migrated from a Chemstation v8 environment. I do a mixture of development work and QC work, often using multiple wavelengths.

The development work is mainly using sequences to obtain blank baselines with some qualitative samples to develop or refine in-process control methods. Lots of unique methods.

I'd like to be able to simply generate simple Chemstation type reports with Area % without having to enter any calibration data, stick example chromatograms into methods, or extensively reprocess the data, as reprocessing is timeconsuming ( and unfortunately currently compromising the software stability ).

My naive playing around, along with some local support, hasn't found any simple method, which is surprising, as I had expected the default sequence reports to easily offer area % for a selected wavelength signal, so I'm sure I'm missing something obvious.

Currently, it's quicker to select and reprocess the peak areas using Excel, rather than play around with calibrations. Any pointers to relevant sections of the manuals, Agilent resources, techniques to use, or any other options using Cerity would be greatly appreciated.

Thanks in advance for any suggestions, and please keep having fun,

Bruce Hamilton

[Tim]

Bruce,

Area% is available by default in Cerity.

To get it to appear on the screen results,:

while on the Method tab, go to Data Review Layout>Single Injection. Click on "Results Table" on the right hand side. There you can select the columns to be displayed from the Available column (select what you want and click > to move to the Display column). There is an "Area% relative to all peaks" and "Area% relative to peaks on signal".

To get the Area% on a single injection report:

while on the Method tab, go to Reporting. Click on Sample Single Injection Report. I will assume you are using the default Inj_short.html report, but should work if you have created some yourself. Click "Edit Template" to launch the report editor. On the right of the screen where the report is shown, scroll down to the bottom of the report to where the peak results are shown. Right click on any column in the table and select "Table Properties". From there you can select any available data field relevant to a single injection. I would suggest selecting one of the Area% fields that appear under "Main Peak" in the selection list - just check the box and click OK.

Cerity will add any new fields to the right side of the table. Unfortunately the only way to change the order (that I have found) is to delete everything and then gradually add them in the order you want them.

For the report change, you should just be able to reprint the reports. For the display change, you will need to reprocess the run usnig the latest version of the method.

Hope that is of some use.

[Bruce Hamilton]

Hi Tim,

Thank you very much for that!. The issues I'm having may relate to how my current methods are set up, and I'll have a further play next week - once I've finished some long sequences, as playing during sequences currently causes problems.

I can follow most of your suggestion except the "Main Peak" choice doesn't appear, but I think I have have seen it previously, so will have a play when the instrument is available - possibly by creating a new method.

The problem may be due to my playing with %area of all peaks on signal" - which wasn't selected from the main peak option, but the from signal option, I think. The resulting report gave % area for all signals recorded eg DAD A 220nm and DAD B 290nm would give 200% total.
I needed to add a Sigmal ID field to determine which line came from which wavelength.

Also, I cleverly managed to get multiple calculations reported based on the number of peaks, eg if the sample contained 3 peaks the table would include 3 complete sets of results for the sample ( 9 lines ), if the sample contained 5 peaks, the table would contain 25 lines.

Unfortunately, my original crude sample contained about 80 peaks, Oops... Global deforestation was accelerated... Maybe starting with a clean method is a good idea.

Thanks for the help, and I will report back, once I've had a good play.

Please keep having fun,

Bruce Hamilton