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Grob Test

http://www.chromforum.org/viewtopic.php?t=3326

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Grob Test

Posted: Thu Jan 26, 2006 9:45 am
by WK
Hi All,
I have just bought a grob test mix and the chromatography I get is good except the 2,3-butanediol. For this I get 2 peaks (the 1st is half the size of the 2nd). They both have the same mass spectra. All grob tests published have one peak for the 2,3-butanediol. Does anyone have the same problem? I am running a RTX5 both on MS and FID and the problem is the same. Injection split 100:1 1mlmin helium 0.1ul inj 250degC.
Best Regards
WK

Posted: Thu Jan 26, 2006 10:04 am
by MikeD
WK

They are the two diastereoisomers. To get one peak you either need to obtain one of the diastereoisomers or the meso- form. I didn't bother - I just used the twin peaks as my column performance reference point.

Posted: Thu Jan 26, 2006 10:08 am
by MikeD
WK

I just realised you said you bought the mix. I made up my own Grob mix and could only blame myself. Where did you buy it ?
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