Protein separations

[rnuijts]

Does anyone if it is possible and if yes with what type of column, to separate two proteins of 144KD and 146KD with HP-SEC. I think it is not possible.

[HW Mueller]

If you find something that does this let me know, the best I ever did is separate (almost baseline) 150 000 from 100 000. Rule of thumb is that one should have about a factor of two diff in molecualar weight, last time I saw. (This excludes some special cases)

[SIELC_Tech]

Rnuijts,

What is the difference in charges of these molecules? We can take this challenge and try to separate these two proteins (free of charge). We had a great success with smaller proteins separated by mixed mode approach.

Let me know if sending a sample is an option for you. We will screen this on our Promix columns for proteins:

http://www.sielc.com/Technology_Peptides.html

Let us know if you would like to use this offer.


Vlad

P.S.
Hans,

If you have some proteins fitting this description may be you can provide us with a sample. I am curious to try it.

[Kostas Petritis]

If you do not care about mainting the bioactivity of your proteins, I would suggest you to try the macroporous column from Agilent (i.e. mRP).

Not quite what you asked but thought to suggest an alternative. About your question, I do not think you can do it with SEC (as Hans pointed alerady out).

[rnuijts]

Thank you for your replies. I will take them in concideration.

[HW Mueller]

Vlad, the expressed interest, above, was limited to SEC columns and possible secondary phenomena of them.
As mentioned before, the materials that would be of interest for columns not in my possesion can not be send too far: Short lived radioactivity. Thanks anyway, maybe ............