Analysis of xanthine hypoxanthine and related compounds

[philippem]

Hallo,
Does anybody has experience with the analysis of xanthine, hypoxanthine, guanine and adenine by means of HPLC.

We 've used an YMC ODS-A C 18 column , but we observe a changing selectivity per column batch?

Supelcosil LC 18-T C 18 column did not resolve all the peaks of interest

Who has experience with other column brand(s)

regards

Philippe

[SIELC_Tech]

Here is our method for guanine and related compounds:

http://www.sielc.com/compound_050.html

Here is information on the column:

Primesep 200 is a reverse-phase analytical column with embedded weak acidic ion-pairing groups.

Improves retention of strong basic compounds by cation-exchange mechanism.
Retains neutral compounds by reverse-phase mechanism.
Provides an additional interaction with polar molecules offering separation of isomers and structurally-related compounds.
All mobile phases are LC-MS and preparative chromatography compatible.

Contact us if you have more questions.

regards,

Vlad