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siRNA separation
Posted: Wed Jul 13, 2016 11:12 am
by weeker2001
the duplex of siRNA
my iclass
column temperature 25
TEAA 100mM
mobile phase A
mobile phase B 20% A in ACN
gradient elution 35% --85%
Re: siRNA separation
Posted: Fri Jul 15, 2016 2:47 am
by weeker2001
anyone got the experience of oligonucleotide analysis ?
Re: siRNA separation
Posted: Fri Jul 15, 2016 1:40 pm
by Andy Alpert
Typically one analyzes single-stranded oligonucleotides smaller than ~ 18 bases by reversed phase and by anion-exchange if they're larger. Anion-exchange is the method to use for double-stranded oligos. What exactly are you trying to separate from what?