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- Posts: 11
- Joined: Sat Dec 07, 2013 11:08 am
One of my clients has produced a validation protocol for an SEC column changeover.
In their proposed testing protocol they have stated that they wish to change the storage solution from the manufacturer’s recommendation (20% Ethanol) to our working buffer (a saline formulation buffer) with a little Sodium Azide.
Their justification is as follows:
- Reduced flushing times as we will no longer need to flush with water prior to or after storage solution.
- Longer column life as it will avoid contraction and expansion of the agarose gel beads caused by the change in solvents.
I was wondering if this is common practice? Has anybody had any experience with this and does it help?
In their proposed testing protocol they have stated that they wish to change the storage solution from the manufacturer’s recommendation (20% Ethanol) to our working buffer (a saline formulation buffer) with a little Sodium Azide.
Their justification is as follows:
- Reduced flushing times as we will no longer need to flush with water prior to or after storage solution.
- Longer column life as it will avoid contraction and expansion of the agarose gel beads caused by the change in solvents.
I was wondering if this is common practice? Has anybody had any experience with this and does it help?
