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Dry Prime without flow
Posted: Tue Jun 14, 2016 7:07 pm
by Dalyara
Hi!
I´m using a Waters Alliance e2695. I have tried to perform a Dry Prime but no mobile phase is coming out. It seems that mobile phase is not being pulles from the reservoir.
I've already tried to repeat the procedure but the two lines remain without flow.
Someone had the same problem?
Re: Dry Prime without flow
Posted: Tue Jun 14, 2016 7:52 pm
by tkubowicz
Hello
You need to pull liquid manually with syringe (through the valve).
Regards
Tomasz Kubowicz
Re: Dry Prime without flow
Posted: Wed Jun 15, 2016 1:28 pm
by Dalyara
Hi, Tomasz!
I'm using the syringe to pull the liquid, even though the mobile phase is not coming out.
I've already checked the reservoir filters and they're not blocked. Seems to be another problem.
Re: Dry Prime without flow
Posted: Wed Jun 15, 2016 4:09 pm
by DR
Make sure you're pumping a wet line (actually, they should all be wet).
Open the valve lots, make sure you've got a good seal w/ the syringe and...
If at first you don't succeed, keep sucking.
You'll eventually get it going.
Re: Dry Prime without flow
Posted: Thu Jun 16, 2016 2:36 am
by tom jupille
Also, make sure that the "sinker frit" in the reservoir is not clogged and that you don't have any kinks in the inlet line(s).
Re: Dry Prime without flow
Posted: Thu Jun 16, 2016 6:50 pm
by uzman
Seems like inlet checkvalves stuck.
Ultrasonicate the checkvalves in methanol and try again.
Re: Dry Prime without flow
Posted: Thu Jun 16, 2016 7:49 pm
by James_Ball
Seems like inlet checkvalves stuck.
Ultrasonicate the checkvalves in methanol and try again.
I am not familiar with the Waters pump, but if the inlet and outlet check valves can be swapped, make sure they are not reversed. I had a pump on and IC before where they were installed reversed and definitely nothing will flow lol.
Re: Dry Prime without flow
Posted: Fri Jun 17, 2016 12:33 pm
by DR
Arrows should always point up for Alliance system check valves.