By lucia on Tuesday, November 18, 2003 - 09:35 am:

I´m trying to implement a method for the determination of sulfonamides in honey, but the five standars have very close retention times which make them imposible to resolve, is there any possibility without changing the mobile phase?

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By Anonymous on Tuesday, November 18, 2003 - 04:49 pm:

Lucia,

It is always best to play with the mobile phase to get better resolution. However, you may get an improvement, if you reduce the flow rate by a factor of two (and wait two times longer for the peaks to come out).

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By lucia on Wednesday, November 19, 2003 - 06:00 am:

supossed the same response and the noise is not a problem, which one is more desirable?
gain 18 volume 20ul
gain 10 volume 100ul

In other words, is there any extra expense of the detector using higher gains?
thank you in advance