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to make c-18 reverse phase column
Posted: Wed Dec 14, 2005 8:46 am
by sunmm
hello everyone!
now i make c-18 reverse phase column myself,but the presuer was so high .i do'nt know why ! is here someone know some method to make c-18 reverse phase column!
thank you ,i apreciate your anwser!
Posted: Wed Dec 14, 2005 11:43 am
by oalamu
What exactly do you mean ,are you using RP-C18 for your chromatographic analysis or you manufacture your own C-18 column?
Posted: Wed Dec 14, 2005 12:37 pm
by philippem
Hi there,
If you want to pack your own HPLC columns ( stainless steel columns) you need special equipment which is probably not available in your lab I'm afraid.
You need a constant pressure pump ( up to 1000 bar) and filing vessel capapble of handling the high pressure.
If you want to pack minibore columns ( ID less 2mm) you could use a constant flow pump, any HPLC pump will do and try to fill these columns.
However it is a particular job and a lot of experience and proper proceuders have to be followed.
This knowledge is protected by column manufacturers.
Regards
Philippe
Re: to make c-18 reverse phase column
Posted: Thu Dec 22, 2005 10:03 am
by amaryl
hello everyone!
now i make c-18 reverse phase column myself,but the presuer was so high .i do'nt know why ! is here someone know some method to make c-18 reverse phase column!
thank you ,i apreciate your anwser!
How did you pack your column? Who suggested you instead of analysis manufacture column
Regards,
Amaryl.