to make c-18 reverse phase column

[sunmm]

hello everyone!
now i make c-18 reverse phase column myself,but the presuer was so high .i do'nt know why ! is here someone know some method to make c-18 reverse phase column!
thank you ,i apreciate your anwser!

[oalamu]

What exactly do you mean ,are you using RP-C18 for your chromatographic analysis or you manufacture your own C-18 column?

[philippem]

Hi there,

If you want to pack your own HPLC columns ( stainless steel columns) you need special equipment which is probably not available in your lab I'm afraid.

You need a constant pressure pump ( up to 1000 bar) and filing vessel capapble of handling the high pressure.
If you want to pack minibore columns ( ID less 2mm) you could use a constant flow pump, any HPLC pump will do and try to fill these columns.

However it is a particular job and a lot of experience and proper proceuders have to be followed.

This knowledge is protected by column manufacturers.

Regards

Philippe

[amaryl]

hello everyone!
now i make c-18 reverse phase column myself,but the presuer was so high .i do'nt know why ! is here someone know some method to make c-18 reverse phase column!
thank you ,i apreciate your anwser!

How did you pack your column? Who suggested you instead of analysis manufacture column

Regards,

Amaryl.