re: HPLC pump flow

[Renuka]

I am working with the reversed phase HPLC and gradient method. My hplc pump was running fine before. It has done approx600samples but now sometimes there is no flow from the pump but the pump is on and the light glows.

Do you have any answer why this si happening

thanks

[ravenwork]

Obvious things to check:

1) Is mobile phase reaching the pump? available? lines crimped or blocked? inlet filter dirty?

2) Is the pump primed with working check valves?

[jitender]

Check what Raven suggested:

1. If pressure exceeding too much; may be blocked line somewhere.
2. If no pressure build up at all; Pump may not be primed. Suck the mobile using the syringe provided.
3. if pressure fluctuating; may be some air entraped in-line.


Most apparent cause to your problem is Pump not primed.