Chromatography Forum

Hi everyone,

Iam new to GS, recently I changed column and in the guideline it says to confirm if its correctly installed. I use recommended method but when I press "start" peak appears by itself without injecting sample, and when I inject it nothing happens. What could be the cause? Thank you in advance.

Hi everyone,

Iam new to GS, recently I changed column and in the guideline it says to confirm if its correctly installed. I use recommended method ...

Let us know what exactly have you done to "confirm".

Hi everyone,

Iam new to GS, recently I changed column and in the guideline it says to confirm if its correctly installed. I use recommended method ...

Let us know what exactly have you done to "confirm".

Thank you for you response.

For inlet:
Mode - split
Heater - 40C
Pressure - 20 psi
Split ratio - 5:1

Oven temp 40C

Detector:
Heater 200C
Reference flow 30

after I injected butane I got a peak I needed but its up side down (it looks down instead of up) how can I change it?

And the type of detector is ...
Is your column capillary or packed ?

And the type of detector is ...
Is your column capillary or packed ?

its TCD and its capilary

And the type of detector is ...
Is your column capillary or packed ?

its TCD and its capilary

Is it possible to change the polarity of the signal to get positive peak ?

And the type of detector is ...
Is your column capillary or packed ?

its TCD and its capilary

Is it possible to change the polarity of the signal to get positive peak ?

Great, thank you so much dblux!
I did it and got a positive peak, I just wondering is it considerate as a right way?

its TCD and its capilary

Is it possible to change the polarity of the signal to get positive peak ?

Great, thank you so much dblux!
I did it and got a positive peak, I just wondering is it considerate as a right way?

Another question, what kind of signal plot should I chose? front detector or front column 1?

its TCD and its capilary

Is it possible to change the polarity of the signal to get positive peak ?

Great, thank you so much dblux!
I did it and got a positive peak, I just wondering is it considerate as a right way?

It is the right way to get positive peaks by chooseing appropriate polarity.

Is it possible to change the polarity of the signal to get positive peak ?

Great, thank you so much dblux!
I did it and got a positive peak, I just wondering is it considerate as a right way?

Another question, what kind of signal plot should I chose? front detector or front column 1?

If your detector is installed in front channel then you should choose a signal from front detector.

Great, thank you so much dblux!
I did it and got a positive peak, I just wondering is it considerate as a right way?

Another question, what kind of signal plot should I chose? front detector or front column 1?

If your detector is installed in front channel then you should choose a signal from front detector.

Thank you for your answers!!!
The guideline says I need to condition the column and only oven temperature was given, do you know by any change what are other parameters?

Try to "google" column conditioning on manufacturers website. The most important thing is to have carrier gas flow during conditioning. Don't stress the column with too high temperature and too long conditioning.