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Choise of guard-column

Posted: Mon Mar 14, 2016 9:28 pm
by hplcnewuser1
Hi, I am using styrene/divinyl benzene, polymer PLPR-S 250X4.6 8um and I believe it is clogged. What is a good guard column for this column. We are separating peptides. Any suggestion appreciated.

Re: Choise of guard-column

Posted: Mon Mar 14, 2016 10:30 pm
by sav
Its general recommendation: PLPR-S, 8um, 10-20mm X 4.6 or 4mm ID

You can also use only precolumn filter (PEEK or Ti) - for example, VICI Sure-Guard In-Line Filter (Ti, 0.5 or 2um).

P.S.: Also you must verify your beliefs ;)