Chemstation: how to optimize data processing (LC-UV/FLD)

[odt]

We use an Agilent 1290 system for the separation of blood plasma samples and we usually have well separated peaks. However, when we analyze a sample, we still have a lot of reprocessing work to do. The chemstation software integrates every small peak on the chromatogram and sometimes the baseline in the UV-chromatogram is really poor, resulting in a bad integration. Consequently, we need to delete the automated integration and then we manually integrate each peak of interest.

This is quite time-consuming but I don't find how to optimize this data processing.
In fact, I would like to have:
- less peaks integrated
- maybe a time-window in which the software can integrate, so the baseline is not influencing the integration

I have already found how to change some of these parameters after a sample has run, but then I need to change these parameters for each sample. It would be easier if this can be automatically done.

Is there someone who can help me to figure this out?
I thank you!

[bundy5555]

Could you please upload a chromatogram.

[itspip]

You may need to change the threshold for defining a peak. It would be in the the method setup somewhere. Alternatively, you could turn off integration and manually add the peaks of interest.

[Consumer Products Guy]

What I'd do was to load the file of a "difficult" injection, and optimize the Integration Events for it. For example, if only peaks about 5 minutes are of interest, turn off the Integrator at time zero, and then back on again at 6 minutes. Then use the other integration functions like slope sensitivity, threshold, Peak Area, etc. to make the integration how you like it, and then save the Method. Older revisions of Chemstation will have Threshold, newer Slope sensitivity, see if your changes make integration better, worse, or the same. Then do a Calibration Table using your standard. Save the Method again.

[ATAlR]

We use an Agilent 1290 system for the separation of blood plasma samples and we usually have well separated peaks. However, when we analyze a sample, we still have a lot of reprocessing work to do. The chemstation software integrates every small peak on the chromatogram and sometimes the baseline in the UV-chromatogram is really poor, resulting in a bad integration. Consequently, we need to delete the automated integration and then we manually integrate each peak of interest.

This is quite time-consuming but I don't find how to optimize this data processing.
In fact, I would like to have:
- less peaks integrated
- maybe a time-window in which the software can integrate, so the baseline is not influencing the integration

I have already found how to change some of these parameters after a sample has run, but then I need to change these parameters for each sample. It would be easier if this can be automatically done.

Is there someone who can help me to figure this out?
I thank you!

In Data Analysis you can modify integration parameters like specify integration on and off times, area reject, high reject and many others. When you are satisfied with result just overwrite the old method, so the new run will automatically analyze with new parameters .