Chromatography Forum

Chromatography Forum is a public discussion group where you can post questions, news, or messages of interest to chromatographers everywhere, but you must be registered to participate (registration is FREE). If you are a registered user, please log in.
http://www.chromforum.org/

Waters 2487

http://www.chromforum.org/viewtopic.php?t=27936

Page 1 of 1

Waters 2487

Posted: Sat Jan 23, 2016 5:20 pm
by fse7596
Hi,
I have a problem with Waters 2487 detector. The base line is smooth around 250 nm (UV wavelenghts), but when set to 550nm , it shows regular spikes every exactly 20 seconds. No matter what flow rate, mobile phase or gradient.

Re: Waters 2487

Posted: Tue Jan 26, 2016 12:36 pm
by danko
It would’ve been best if there was a chromatogram/data to illustrate the problem, but it’s most probably the lamp that's about to fale. If the spikes became more frequent with a faster flow-rate then it could've been an air bubble cycling in the flow-cell.

Best Regards
All times are UTC
Page 1 of 1
Powered by phpBB® Forum Software © phpBB Limited
https://www.phpbb.com/