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Calculation of LOD when there is caryover in the blanks

http://www.chromforum.org/viewtopic.php?t=27661

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Calculation of LOD when there is caryover in the blanks

Posted: Wed Nov 11, 2015 12:03 pm
by csousa
the s/n of the peak in the blank was subtracted from the s/n of the peak in the 0.01% std injection
Is this the typical way to calculate LOD if a peak is observed in the blank injection or should the peak height of the blank have been subtracted from the peak height of the 0.01% std before calculating the s/n?

Re: Calculation of LOD when there is caryover in the blanks

Posted: Wed Nov 11, 2015 12:23 pm
by Peter Apps
Welcome to the forum.
the s/n of the peak in the blank was subtracted from the s/n of the peak in the 0.01% std injection
Is this the typical way to calculate LOD if a peak is observed in the blank injection or should the peak height of the blank have been subtracted from the peak height of the 0.01% std before calculating the s/n?
The peak in the blank is noise. If you are going with the usual optimistic S:N > 3 criterion for LOD, then the peak from the standard must be > 3 x (blank peak + baseline noise).

Peter
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