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Online high base
Posted: Thu Oct 08, 2015 11:00 pm
by josefina19
Baseline chromatography rises at the end of injection. I have a sample running 6.0 minutes a peak at RRT 2.5 and 3.2 and another 5 minutes as a square (not peak) me on the chromatogram. What could this be? Appreciate your comments. Thank you
http://i59.tinypic.com/2excab7.gif
Re: Online high base
Posted: Fri Oct 09, 2015 6:18 am
by kubowicz.tomasz
Hello
Please paste chromatogram - instruction below:
viewtopic.php?f=1&t=2617
And also please put information about your method:
-parameters
-analyzed compounds
-sample (solvent)
It is the best way to have clear picture what your problem is
Regards
Tomasz Kubowicz
Re: Online high base
Posted: Mon Oct 12, 2015 8:38 am
by Gerhard Kratz
Without information I can only guess: you are running a gradient?
Information on the mobile phase and composition of MP would be highly appreciated.
Re: Online high base
Posted: Mon Oct 12, 2015 10:36 am
by Peter Apps
With a detection wavelength of 205 nm it could be almost anything.
Peter
Re: Online high base
Posted: Tue Oct 13, 2015 6:39 am
by kubowicz.tomasz
Hello
Please provide more details (I mentioned that in first post).
I suspect that you run gradient and when peak elution in 5.3min starts, gradient is changing to initial settings.
Regards
Tomasz Kubowicz