CMC enzymatic degradation products
Posted: Fri Jul 31, 2015 3:33 pm
Hello everyone.
I will appreciate help on the chromatographic analysis of engoglucanase activity on carboxymethil cellulose using an oligosaccharide resex column (Phenomenex).
The enzymatic assay is preformed using 2%v (w/v) of CMC and I have problems separating the products of the enzymayic reaction.
I use a microbial enzyme and I need to separate the CMC from the degradation products before loading the sample.
THANK YOUUUU
PAOLA
I will appreciate help on the chromatographic analysis of engoglucanase activity on carboxymethil cellulose using an oligosaccharide resex column (Phenomenex).
The enzymatic assay is preformed using 2%v (w/v) of CMC and I have problems separating the products of the enzymayic reaction.
I use a microbial enzyme and I need to separate the CMC from the degradation products before loading the sample.
THANK YOUUUU
PAOLA