Low Response on Dionex Electrochemical Detector (ICS-3000)

[dangdk]

Hi,

We acquired an old (2005) Dionex ICS-3000 Detector Compartment (DC unit). We also have an existing ICS-3000 system with DC that we have used since 2008.

We are trying to get similar responses on the old-system electrochemical detector to what we get on our existing system. However, our peaks for sugars (monosacharides: glucose, xylose etc.) are tiny compared with the existing system. We have tried all different combinations of:

- Cells (including one taken from the existing system).
- Working electrodes (including one taken from the existing system).
- Reference electrodes (including one taken from the existing system).
- Calibrating the reference electrode.
- We also took the cell, working/reference electrodes of the old system and put them in our existing one and the peak heights/areas came out fine.
- Whole electrochemical detector units (including one taken from the existing system). Note that for the old system we acquired the DC was labelled 2005 but the ED inside it was labelled 2010, however the one taken from the existing system was from 2008.
- Position the ED unit was placed in the DC unit (left or right, i.e. system 1 or system 2).
- Different pumps.
- Different columns.
- Injection valves (the left and the right valve in the DC unit).
- Avoiding columns completely, and just loading the loop with sugar solution and then passing that directly to the detector. In such a case we would expect a massive peak of height around 1000 but it is only around 15.
- We have gone to the server config and looked at the configuration of the ED in the old DC unit and the settings in the "signals" tab are the same as for the existing DC unit.

The old system is a second timebase with the first system running on timebase 1. We are using Chromeleon 6.80 SR10 Build 2818.

Please help we are losing our minds with this

Thanks

Dan

[John Guajardo]

Hi Dan,

What model of autosampler do you have on each system (or are they sharing one sampler with a diverter valve)? Any chance you can make Chromeleon backup files of the data and email the resulting *.cmb files to me (john.guajardo@thermofisher.com)?

[dangdk]

Hi john,

I sent you an email with the cmb file. We use an AS50 on both systems (one each), there is no diverter valve.

Thanks

[dibyendughosh]

Hi Dan,

Is this problem resolved??

If no then there would be Auto Sampler setting issue.

Let us know we will go deeper into the Auto Sampler settings, to sort out your problem.

Dibyendu Ghosh
Customer Care Manager
Thermofisher Scientific