Best mobile phase for Catecholamine HPLC

[Cshahr]

I am very new with HPLC . I am working with DIONEX /thermo sytems.

I would like ask folks who are working on Catecholamine HPLC or have experience.

Can anybody help with basic setting?
specially I would like to make sure that what kind of mobile phase is the best mobile phase?


Thanks

[artsjeroen]

Which catecholamine do you want to analyze? And what kind of detector do you want to use?

With ECD detection:
http://www.myantec.com/markets-and-solu ... cholamines

[mattmullaney]

Agreed with artsjeroen, Google Scholar also lists quite a few papers with a variety of detectors and separation conditions for catecholamines.

Thermo's own website has a good example or two, too.

http://www.dionex.com/en-us/webdocs/844 ... 269-R2.pdf

Determination of Catecholamines in Human Plasma by Liquid Chromatography with Electrochemical Detection

http://www.sciencedirect.com/science/ar ... 7311017936

Abstract
This study involved three commercial reversed-phase (RP)/anion-exchange (AEX)/cation-exchange (CEX) trimodal columns, namely Acclaim Trinity P1 (Thermo Fisher Scientific), Obelisc R (SIELC Technologies) and Scherzo SM-C18 (Imtakt). Their chromatographic properties were compared in details with respect to hydrophobicity, anion-exchange capacity, cation-exchange capacity, and selectivity, by studying retention behavior dependency on organic solvent, buffer concentration and pH. It was found that their remarkably different column chemistries resulted in distinctive chromatography properties. Trinity P1 exhibited strong anion-exchange and cation-exchange interactions but low RP retention while Scherzo SM-C18 showed strong reversed-phase retention with little cation-exchange and anion-exchange capacities. For Obelisc R, its reversed-phase capacity was weaker than Scherzo SM-C18 but slightly higher than Trinity P1, and its ion-exchange retentions were between Trinity P1 and Scherzo SM-C18. In addition, their difference in selectivity was demonstrated by examples of determining the active pharmaceutical ingredient (API) and counterion of drug products.

Please, see what you think and thank you!