decanesulfonic
Posted: Sun Mar 29, 2015 7:07 pm
by gerapas
Hi, is it possible that a specific c18 column 100x4.6 needs several hundrends of column volumes to be conditioned( no retention time drift) when m.phase has decanesulfonic acid for IP(5mM)? It took us 2 days with flow rate 2, to have stable RT. we tested 2 columns of the same brand.
Re: decanesulfonic
Posted: Sun Mar 29, 2015 9:45 pm
by tom jupille
Yes, it is quite possible. Going back to the archives:
http://www.lcresources.com/discus/messa ... 20010652am
Re: decanesulfonic
Posted: Mon Mar 30, 2015 7:16 pm
by Vlad Orlovsky
just use 20 times more acid for initial saturation and you should be able to do this in an hour or so.