Setting Up Quantitative Analysis

[LallyG]

Agilent Chemstation E.02.02.1431

I ran 5 standards to set up a calibration table via GCFID.

I tried following the instructions I found here http://www.chem.agilent.com/Library/Sup ... a05214.pdf, but I seem to be missing something. This set up seems to be tailored to MS. I made sure to unclick "Use RTEINT" in the "Set Up Quantitation" box. All compounds have been inserted. After this step, I'm at a loss. I added levels. I used "QEdit Quant Results" to get responses for each peak. Entered the "concentration" and "response" values for each compound. Then, when I selected "Quantitate" > "Calculate" nothing. There's something I'm missing. What makes the FID quantitation set up different from MS. Certain icons, like "AutoQuant Setup" is for MS Data Only.

I get no response for AutoIntegrate or Integrate either. Can anyone point me in the right direction, please?

[Steve Reimer]

The E version is set up for MS. It sounds like you aren't integrating any peaks. Normally you would enter the expected retention times and the concentrations, when you process a file with a standard Chemstation will integrate the peaks and you will update the calibration level. Chemstation then assigns the responses for the identified peaks to that claibration level. If it has no assigned peak you have no update.
I haven't tried an FID with this software so I'm guessing here but if you look in edit compounds is the quant signal set to gc1 or gc2 (or TIC?) rather than target ion? If it doesn't integrate anything check out the integration parameter file.

[LallyG]

The E version is set up for MS. It sounds like you aren't integrating any peaks. Normally you would enter the expected retention times and the concentrations, when you process a file with a standard Chemstation will integrate the peaks and you will update the calibration level. Chemstation then assigns the responses for the identified peaks to that claibration level. If it has no assigned peak you have no update.
I haven't tried an FID with this software so I'm guessing here but if you look in edit compounds is the quant signal set to gc1 or gc2 (or TIC?) rather than target ion? If it doesn't integrate anything check out the integration parameter file.

Thank you so much for responding,

Quant signal is blanked out but lists TIC.

I checked the integration parameter file and updated a few things:
Integrator Event Name - Value - Time:
Initial Area Reject - 1 - Initial
Initial Peak Width - 0.020 - Initial
Shoulder Detection - OFF - Initial
Initial Threshold - 11.5 - Initial
Integrator OFF - N/A - 0.001
Integrator ON - N/A - 3.000

This seemed to work after I selected Calibrate >> Integrate.

I'm not very familiar with Integration Parameter (this seemed to solve the issue), what are the basics? Where can I find a Chemstation for Dummies book? Ha!

Thank you so much Steve Reimer!