Chromatography Forum

It has been several years since I have worked with the chemstation software for an Agilent HPLC. When the detector (Fluorescence) turns on it goes from 0 up to 17 on the y axis and that is where it stays during the analysis. I would like to set it to read at 0. I know it does not change the chromatography any, but I would still like it at 0. I've looked on line and did not see anything.

It looks like you can add a baseline zero event in the timetable under the FLD method parameters.

Depending on the specifics of your method, a baseline that high might be caused by light scattering from a dirty flow cell or optics, but as you said, that should not affect your chromatography if the baseline is stable.