GC method development

[yara]

how to start method development in gc?? i read agilent's presentation on this topic but still don't know where to start??how to choose suitable column to my substance??what rule controls usage of pressure control or flow control of gases in gc ,i mean when to choose pressure or to choose flow control?? how to set up the value of either pressure or flow required to my method,thanks in advance

[Steve Reimer]

If your proposed analysis is similar to one that Agilent (or another vendor) has a technical note for use the conditions listed in the note as a starting point.

[yara]

i read agilent's note on method development but still cann't find answers to the previous questions

[Peter Apps]

1. You first need to know how to run the GC. If you have nobody to train you, then look on the vendors websites for instructions and manuals.

2. When you know how to run the GC do some routine analysis with established methods so that you become familiar with it.

3. When you want to develop a new method look for application notes from any of the vendors, what works on a Thermo will work on a Bruker will work on an Agilent if you know how to run the GC (see #1).

4. If there are no application notes that are helpful, then do a search of the scientific literature. You will probably have to adapt these method slightly to get them to work optimally.

Peter