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- Posts: 2
- Joined: Thu Oct 23, 2014 8:54 am
Hi everybody, I'm writing here because I found this forum really useful and hope you can help me. First of all sorry for my bad english!
I recently had a problem on my 5973 MS: after a routinary cleaning of ion source, I tryed doing the autotune as usual and I had a problem of corrupted file, so I loaded an old one that I knew it was correctly working and it seemed to be all ok.
The problem is that, when I start my analysis (4Methyl imidazole on balsamic vinegar) in SIM mode, I do see the internal standard but can't see the 4MeI!I also thought it could be a standard problem, so I bought a new one but nothing changed.
I did a different analysis (glicols) and the areas of the standards newly prepared correspond to the expected values that I obtained in previously calibration....
what can be the cause of this problem? Which test do you suggest to verify the effective functionality of the MS spectrometer?
I'm really upset because I can't understand where the problem could be! I never did a manual tune but I dont' think this could be the problem because, in my opionion, if the mass doesn't work, it doesn't work everytime and not only with some substances... no analytical changes in the method, no change of ramp temperature, column, flow, I did in the past the same analysis and every time worked great... could the column have completely lost the affinity for this substance? I can do a change with a similar column (FFAP instead CPWAX) and do another test meanwhile I wait for some help from you all.
Every suggestion will be really helpful.
Have a nice day to everybody and thank you very much.
babs'
I recently had a problem on my 5973 MS: after a routinary cleaning of ion source, I tryed doing the autotune as usual and I had a problem of corrupted file, so I loaded an old one that I knew it was correctly working and it seemed to be all ok.
The problem is that, when I start my analysis (4Methyl imidazole on balsamic vinegar) in SIM mode, I do see the internal standard but can't see the 4MeI!I also thought it could be a standard problem, so I bought a new one but nothing changed.
I did a different analysis (glicols) and the areas of the standards newly prepared correspond to the expected values that I obtained in previously calibration....
what can be the cause of this problem? Which test do you suggest to verify the effective functionality of the MS spectrometer?
I'm really upset because I can't understand where the problem could be! I never did a manual tune but I dont' think this could be the problem because, in my opionion, if the mass doesn't work, it doesn't work everytime and not only with some substances... no analytical changes in the method, no change of ramp temperature, column, flow, I did in the past the same analysis and every time worked great... could the column have completely lost the affinity for this substance? I can do a change with a similar column (FFAP instead CPWAX) and do another test meanwhile I wait for some help from you all.
Every suggestion will be really helpful.
Have a nice day to everybody and thank you very much.
babs'
