Cleaning Columns?
Posted: Tue Oct 21, 2014 5:15 pm
by Harpo
I'm taking a chance on some used columns ($40 vs $700!), and wonder if there is a reliable way to clean them.
They're Waters Symmetry C8 4.6x150mm, mobile will be MeOH/AcCN/water/HOAc.
Any good ideas?
Thank you!
Re: Cleaning Columns?
Posted: Tue Oct 21, 2014 7:34 pm
by uzman
If you are mentioning about buying used columns around $40 , please also consider blocked frits , void packing , leaking connections , precipitated buffers inside the column etc.
If you are lucky , and get a column in good condition , then try 20% ACN + 80% water first , and continue with 100% ACN.
Reverse the column during washing , to prevent heavy contamination run into the column.
Re: Cleaning Columns?
Posted: Wed Oct 22, 2014 6:28 am
by Gerhard Kratz
In addition to Uzman please disconnect the detector when rinsing your column.
Re: Cleaning Columns?
Posted: Fri Oct 24, 2014 8:06 am
by mattmullaney
Hello All,
This article is So Good, I've posted this on LinkedIn as well:
http://www.pharmtech.com/pharmtech/data ... rticle.pdf
Thank You, LC-GC magazine!