Cleaning Columns?

[Harpo]

I'm taking a chance on some used columns ($40 vs $700!), and wonder if there is a reliable way to clean them.

They're Waters Symmetry C8 4.6x150mm, mobile will be MeOH/AcCN/water/HOAc.

Any good ideas?

Thank you!

[uzman]

If you are mentioning about buying used columns around $40 , please also consider blocked frits , void packing , leaking connections , precipitated buffers inside the column etc.

If you are lucky , and get a column in good condition , then try 20% ACN + 80% water first , and continue with 100% ACN.
Reverse the column during washing , to prevent heavy contamination run into the column.

[Gerhard Kratz]

In addition to Uzman please disconnect the detector when rinsing your column.

[mattmullaney]

Hello All,

This article is So Good, I've posted this on LinkedIn as well:

http://www.pharmtech.com/pharmtech/data ... rticle.pdf

Thank You, LC-GC magazine!