Chromatogram Peak Issue (Flat Peaks)

[khalidniazkhan]

Hi,

We are using Sykam HPLC with S
The software is "Clarity". During analysis run, when the peak height overs 100 mV, the "OVER" message appears on computer display and the peak gets flat. With this, we are having no sharp peaks and are having large peak area.
Please advise a solution.

Regards
Khalid

[JMB]

It sounds like you have exceeded the linear range of the detector, and therefore have a saturated signal.

Inject x10, x50, x100 dilutions etc. of your sample until you obtain a Gaussian peak (not flat-topped).

[M_Farooq]

Hi,

We are using Sykam HPLC with S
The software is "Clarity". During analysis run, when the peak height overs 100 mV, the "OVER" message appears on computer display and the peak gets flat. With this, we are having no sharp peaks and are having large peak area.
Please advise a solution.

It is clear that you are overloading your column with larger volume of injection. This particular phenomenon is called volume overload. Try to decrease the inj volume + concentration.

[ScottHorn]

Also, check your method, in the "Acquisition" tab. Make sure the "range" setting is identical, or at least close to the range your detector outputs.