HPLC method for HPMCP

[AleksKab]

Hey everybody,

I am a absolute beginner as for HPLC but would like to detect the polymer HPMCP HP55. Recently I read an article which describes the simultaneous detection of API and polymer of the samples in one measurement. I want to follow this strategy but with another system. The API is easily detectable but I have issues to measure a signal for the polymer.

Does anyone know or even has experience with detecting HPMCP HP55 or HPMCP in general?

I'm excited receiving answers from you guys.

Kind regards,
Alex

[JMB]

To separate an API (MW ~200-500) from your hydroxypropylmethylcellulose (??) polymer (MW ~5-10 K) requires an SEC column I assume.

Since HPMCP has no appreciable chromophore you will have to use a refractive index detector for sensitivity.

Please provide further details of the method that you are following.