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Standard Curve

Posted: Mon Jul 28, 2014 2:49 pm
by Gracinha
Hi, I have been having trouble with an extraction, although the calibration curve is linear the amount extracted from the QC samples is the double the expected value. Any help?

Re: Standard Curve

Posted: Mon Jul 28, 2014 4:22 pm
by skunked_once
The first thing I would do is check everything for some factor of two error (dilution, calculation, etc.).
If this doesn't solve the problem, I would post much more information so that a better solution might be suggested.

Re: Standard Curve

Posted: Tue Jul 29, 2014 2:10 am
by KM-USA
Have you assayed placebo samples, and also assayed spiked placebo samples?