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β-cyclodextrin removal

http://www.chromforum.org/viewtopic.php?t=25036

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β-cyclodextrin removal

Posted: Mon Jun 30, 2014 11:34 am
by gnikol21
Hello to all, im using an HPLC method with β-cyclodextrin in mobile phase. Im washing the column with water:ACN 80 :20 to remove this detergent from the column but the pressure in my column didnt fall at the starting level.Anyone know any other way to get rid off β-cyclodextrin from my column?
Thnx in advance

Re: β-cyclodextrin removal

Posted: Mon Jun 30, 2014 2:50 pm
by tom jupille
Try reverse-flushing the column.
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