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β-cyclodextrin removal

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β-cyclodextrin removal

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gnikol21
Hello to all, im using an HPLC method with β-cyclodextrin in mobile phase. Im washing the column with water:ACN 80 :20 to remove this detergent from the column but the pressure in my column didnt fall at the starting level.Anyone know any other way to get rid off β-cyclodextrin from my column?
Thnx in advance

Re: β-cyclodextrin removal

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tom jupille
Site Admin
Try reverse-flushing the column.
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
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