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Calculating the Stock Solution Concentration for a Surrogate

http://www.chromforum.org/viewtopic.php?t=25021

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Calculating the Stock Solution Concentration for a Surrogate

Posted: Wed Jun 25, 2014 11:56 pm
by Diophineae
Hello there,

I am running a small scale repeatability study on a project using LC-MS/MS and am trying to figure out what concentration I should add to my dry matrix for surrogate recovery. I would like to see my compound of interest at around 0.05 uM, my final resuspension volume is 100 uL and my injection volume is 5 uL.

Thank you so much for any help :D

Re: Calculating the Stock Solution Concentration for a Surro

Posted: Thu Jul 03, 2014 11:05 am
by danko
You need to know which sensitivity you’re operating with. To do so, you’ll need to determine at least 3 concentrations of the compound you want to spike in your sample. Then it’s easy to inter- and even extra- polate to a desired amount.

Best Regards
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