Ethephon

[leon]

Hi all,

Anyone have any experience with analysing Ethephon, a plant growth regulator? Hope you could share the method and experience with me.

[oscarBAL]

Hello Leon, I was trying to do that along long time ago! in Central America, I never though to find someone with the same problem, I am Sorry to desapoint you , But I could not to analyze it, I suposs you you are talking about recidues, because the product, is a very easy and weird titration.
I think that this post should be in GC forum, because the ehtephon residues is analyzed by GC, ussing a derivatization, Ican not remember at this moment, I found and article fron Italy, (if my memory is correct), but I could not to get the same results, if you get some GC procedure maybe I could help!

good luck!
Oscar.

[oscarBAL]

Hello again
here, some abastrarct that I found:

http://www.ncbi.nlm.nih.gov/entrez/quer ... t=Abstract

http://www.inchem.org/documents/jmpr/jm ... 77pr25.htm

There are other methods beside the derivatization, analysing ethylene
after hydrolisis, but you have to remember that the most of the fruits produce ehtylene, during the maduration process.

[Poskrobko Jan]

For comparing tested samples to the technical standard I use:
TSK G2000 SW XL 5microm; 300x7,8 mm + TSK G3000 SW XL 5microm; 300x7,8 mm (TosoHaas) temp. 40oC. Eluent: 0,25M NaCl i 0,01M NaH2PO4; pH=4,5; flow: 1,0 ml/min.
About 100 mg of sample dissolved in eluent in 10 ml measuring flask and heated 30-60 min at water bath (about 60oC).
Detection RI.

[leon]

Hi Poskrobko Jan,

Thanks for sharing the method but I am sorry I cannot really understand it. Is this an LC method? Actually I am looking for an LC method for ethephon residue analysis. I was hoping I could skip the derivatisation step necessary for GC method. Do you have the reference for the full paper for this method?

Hi Oscar,

Thanks Oscar for the info. Actually I am trying to analyse for ethephon in beansprouts. Do you think, besides its use for ripening, can it be used for beansprout production to yield shorter roots?

[Poskrobko Jan]

Leon! There was parameters for LC metod I developed and I use with no problems. Unfortunatell it is not in the paper.
Jan

[oscarBAL]

Hi Leon,
I don't have any idea if you can use ethephon for beansprouts, you can look at this paper:L
http://www.agrocourier.com/medien/allge ... UeS_GB.pdf

Oscar.

[leon]

[quote="Poskrobko Jan"]For comparing tested samples to the technical standard I use:
TSK G2000 SW XL 5microm; 300x7,8 mm + TSK G3000 SW XL 5microm; 300x7,8 mm (TosoHaas) temp. 40oC. Eluent: 0,25M NaCl i 0,01M NaH2PO4; pH=4,5; flow: 1,0 ml/min.
About 100 mg of sample dissolved in eluent in 10 ml measuring flask and heated 30-60 min at water bath (about 60oC).
Detection RI.[/quote]

Poskrobko Jan,

What is RI? What is the sample that u test on using this method? What is the purpose of the heating? Can I have the full detail of the method?

[Poskrobko Jan]

RI is Refractiv Index Detector.
I am working for testing purity of ethephon samples from producers. From Your last posts I see You are talking about determination of traces of ethephon in biological material. If yes, then sorry, there are different problems and I don't think my method is useful!
Jan