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peaks in void volume
Posted: Thu Aug 25, 2005 12:51 am
by cahuset
Any suggestions about why I might have a large peak in the void volume of my LC/MS/MS runs?
Do I have overload and therefore this analyte is not retained?
Posted: Thu Aug 25, 2005 9:37 am
by tom jupille
Need more information in order to comment:
- Are you running a pure standard or an actual sample?
- Are you looking at the total ion chromatogram or a specific ion?
- Do you have a big peak at t0 and then your analyte peak, or just one huge peak starting at t0?
Posted: Thu Aug 25, 2005 4:23 pm
by cahuset
this was actual samples (extracts from biota)
and it was the specific transition (from MS/MS) that had the strange peaks
And there are two peaks, the first one at t0 and the second one at the normal retention time.
Posted: Fri Aug 26, 2005 1:32 am
by Uwe Neue
Please see the note that I just posted to Ericz. It could be that your sample is dissolved in an organic solvent, and your sample migrates through the column unretained.
Posted: Fri Aug 26, 2005 10:10 pm
by Uwe Neue
Or you got still so much junk in your sample that you see every mass in your unretained peak.