peak naming

[jayroseville58]

I'm somewhat confuse on peak naming particularly on injection of mobile phase and injection of diluent. What is the best way to address this issue. THANKS.

[tom jupille]

I'm not sure of the context here. Are you referring to how to set up the data system? Or about official terminology? Or common usage?

[jayroseville58]

I'm not sure of the context here. Are you referring to how to set up the data system? Or about official terminology? Or common usage?

I was referring on how should I properly name my peaks when I'm injecting diluent and mobile phase. How should I know that the peak coming on my diluent injection is just pure diluent or in other way, How should I know that the peak coming from my mobile phase injection is just pure mobile phase and no other peaks might be present such as diluent peak.

Thanks

[danko]

You can inject different (f. ex. increasing) volumes of your diluent and mobile phase, to see whether or not the peaks you observe are proportionally increasing as well.
The mobile phase is not supposed to produce any peaks by the way – other than injection noise/disturbance. So maybe you see carry-over.
Collecting and looking at the peaks’ UV spectra is another good exercise in addition to the proportionality test.
Other actions could be reading the method development report, talking to the guys that developed the method etc.

Best Regards

[jayroseville58]

You can inject different (f. ex. increasing) volumes of your diluent and mobile phase, to see whether or not the peaks you observe are proportionally increasing as well.
The mobile phase is not supposed to produce any peaks by the way – other than injection noise/disturbance. So maybe you see carry-over.
Collecting and looking at the peaks’ UV spectra is another good exercise in addition to the proportionality test.
Other actions could be reading the method development report, talking to the guys that developed the method etc.

Best Regards

"The mobile phase is not supposed to produce any peaks by the way – other than injection noise/disturbance. So maybe you see carry-over."

but in most cases, upon injection of Mobile Phase, there are some peaks coming not visible on diluent injections as well

[danko]

I think – in order to clarify the case - we need some data/chromatograms here.

Best regards

[jayroseville58]

I think – in order to clarify the case - we need some data/chromatograms here.

Best regards

Ok, I will take note of that. I will show chromatograms sooner for great help. Thanks.