Validation of Linearity of an analytical method HPLC
Posted: Tue Apr 01, 2014 6:00 pm
Hi,
In order to validate the linearity of an anlytical method for determination of tyrosine in human plasma, i used spike-and-recovery experiment .
My question is : after injection in hplc of standards solutions ( range of 1 to 20 mg/l) . i check the linearity of the curve , R2=0,999 and the equation of regression line : y=1.9 x+ 0.3.
Should i calculate the concentration of spiked plasma using the equation of standard solutions .
Thank You.
In order to validate the linearity of an anlytical method for determination of tyrosine in human plasma, i used spike-and-recovery experiment .
My question is : after injection in hplc of standards solutions ( range of 1 to 20 mg/l) . i check the linearity of the curve , R2=0,999 and the equation of regression line : y=1.9 x+ 0.3.
Should i calculate the concentration of spiked plasma using the equation of standard solutions .
Thank You.
