Components for Waters 2487 DAD

[yoyooligo]

We have a Water 2787 Dual Wavelength detector, what is a collection unit that works well with it and where can I find it used?

[JGK]

Unit is usually used with a Separations module (2695 or 2795) and a PC running empower software

[juddc]

JGK is correct. They also integrate well with older Waters component systems, which can be had very inexpensively, are exceptionally reliable, and are very easy to service, but you'd best not be worried about having a significant system volume. They can provide excellent quality data with modern column packings in the appropriate dimensions. I typically use columns with a 2.6uM particle in 4.6mm x 100mm size and can generate nice separations.

I dug parts of my current LC out of a dumpster, other pieces I acquired on eBay, and I've been known to peruse lab equipment auctions.

By collection unit, do you mean fraction collector?

[remesquaddie]

This detector is NOT a DAD, but a variable wavelength detector with two outputs available at different wavelength if desired. It will work well with just about any data reduction system. I use DATAAPEX CLARITY VERY SUCCESSFULLY.
As for fraction collection, again, any HPLC type fraction collection will do.
Hope this helps.

[juddc]

Indeed it's not a DAD, but the OP essentially corrected the erroneous comment with his or her first sentence, which leads me to believe that he or she is aware of what they have.

[yoyooligo]

Painfully very aware...

Anyway, this system is now being controlled by two computers. One for mass lynx and the other for Clarity. Our output on Clarity is in mV. As a beginner, how do I go about configuring the machine to output in a useful unit? How do I calibrate this equipment?

I set the range to 150 something and Hz to 60 because the 2487 manual said it does that and 50. I have no idea what the AUFS unit on the DAD or what sensitivity to put on Clarity. I could probably figure this all out with enough time but I need to be purifying samples in January when they were supposed to be and running...

[ScottHorn]

To configure the input in Clarity, go to the main Clarity window (the one that shows pictures of all your instruments). Choose system, then configuration. On the left-hand list you should see your A/D converter (colibrick, netpad, upad, int7 or int9). Select the channel that the 2487 is hooked up to and click setup. Change the field that says voltage to absorbance, set the unit to AU and the prefix to m (milli AU). You can select a conversion factor for mv to mAU as well. I don't know about the 2487, but I think it's 1 mv/mAU for the 486. Also be sure to set the appropriate value for the mains frequency in your country (50 or 60 hz). As for the range and sampling interval, which you set in the method, I would go with a range of 1250 mv (that's the closest option to the actual range, which is believe is 1000 mv.). For the sampling interval you said you chose 60 hz, which seems a little high to me, unless you're doing some kind of micro-bore UHPLC. I use 10 hz with no problem, but I never have a peak below about 0.3 minutes wide.

[yoyooligo]

You can't configure clarity lite it seems...the company who sold us these refurbished machines screwed us hard every which way.

[ScottHorn]

I've never used Clarity Lite. When you start the program, isn't there a main window with the "system" option as part of the menu bar? The configuration option you're looking for should be under there. If not, I highly recommend consulting the Clarity support forum at http://www.dataapex.com, or emailing support@dataapex.com. I don't see how the software would be usable without that configuration window though. Maybe you're just overlooking it.

Edit: I just looked back at one of your previous posts. When you said the 2487 manual says 'it does 50 and 60 hz', you do realize that means the detector will function on a 50 or 60 hz mains supply (the plug on the wall), right? That has nothing to do with the value you set in the method in Clarity, which is the rate at which the software takes measurements from the A/D converter. Also, unless hardware limitations prohibit it, there's no reason I can see that you can't run both pieces of software on the same computer.

[yoyooligo]

I spent over two hours trying to find "system." Something about a command line I couldn't find either. Maybe, but I'm not gonna be held accountable if it doesn't work at all in the end. Theres a box thats called "control" thats grayed out. I have also since posting realized my mistake on the Hz value. I think the screen on hammering has the sampling rate.

I did find a place to change the unit of the graph but it's not really a conversion I believe. I think I set a relative range in a graph settings somewhere. If you refer to the manual, the page on system configuration has a "FULL VERSION" box on it.

[ScottHorn]

First and foremost, I'd advise you to contact Dataapex with your questions. I do not speak for them in any way, I've just used their software for a lot of different purposes. That being said, I'll offer what assistance I can. In the full version, when you click the icon to start Clarity, you're presented with a window that lists all your instruments. In Clarity Lite I think you only have one. On the top left of this window is a menu bar item labelled "system", under which you can find the "configuration" option. If you don't see that, you may be in the instrument window. To be sure, close all the Clarity windows and start from the beginning. Click the Clarity icon and look for "system" at the top of the window that appears. See the Clarity reference guide here: http://downloads.dataapex.com/documenta ... -guide.pdf
Pages 1-11 contain all the info about getting to the configuration interface. As I've said, I haven't used Clarity Lite, but surely it has to include this functionality.

As far as "command line" options, you shouldn't have to worry about those to do what you need to do. They mostly allow you to automate certain functions. Don't worry about them.

I don't get what you mean by hammering. Sampling rate is set in the method. You can search the forum for recommendations on sampling rate. I seem to remember hearing people recommend a minimum 10-20 samples per your expected peak width. IE, if you've got 30 second wide peaks, 10 hz is plenty. More hz = larger chromatogram file sizes.

You can set a signal range in the chromatogram window that effects how they're scaled, but the important ranges are the ones you set in the configuration window and the method.