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preparative LC system bleeding

http://www.chromforum.org/viewtopic.php?t=24055

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preparative LC system bleeding

Posted: Wed Jan 08, 2014 6:17 pm
by jiangds06
200ml of mobile phase was collected from 21mm column, some system bleeding(white solid, slightly soluble in mobile phase) was found. I just wonder if column bleeding produce these stuff?

Re: preparative LC system bleeding

Posted: Thu Jan 09, 2014 8:36 am
by Gerhard Kratz
My guess is the following:
It is some buffer salt coming off from the column. Column was stored in mobile Phase for a while. Right?
What is your mobile Phase composition?
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