Advertisement
Chromatography Forum

preparative LC system bleeding

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

2 posts Page 1 of 1
Post a reply

preparative LC system bleeding

avatar
jiangds06
200ml of mobile phase was collected from 21mm column, some system bleeding(white solid, slightly soluble in mobile phase) was found. I just wonder if column bleeding produce these stuff?

Re: preparative LC system bleeding

avatar
Gerhard Kratz
My guess is the following:
It is some buffer salt coming off from the column. Column was stored in mobile Phase for a while. Right?
What is your mobile Phase composition?
Gerhard Kratz, Kratz_Gerhard@web.de
Post a reply
2 posts Page 1 of 1
Return to “Liquid Chromatography”

Who is online

In total there are 2 users online :: 1 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am
Users browsing this forum: Ahrefs [Bot] and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

    Gas Chromatography

      Mass Spectrometry