Gradient Program in HPLC
Posted: Fri Jan 03, 2014 4:19 pm
by saipraveen7
Can anyone please provide me the links where I can get detailed information on different gradient curves (Ex. curve 4, curve 6, curve 9 etc.) that can be used in HPLC during a gradient program and their advantages
- P.Sai Praveen
Re: Gradient Program in HPLC
Posted: Fri Jan 03, 2014 5:35 pm
by bisnettrj2
Which brand/model of pump are you using?
Re: Gradient Program in HPLC
Posted: Fri Jan 03, 2014 5:47 pm
by tom jupille
and their advantages
Essentially there are no advantages. Anything you can do with a curved gradient you can do as well (with fewer transfer problems) using a series of linear segments. The curved gradient profiles are a relic from 40 years ago in the days before digital electronics (curved profiles are easier to generate with analog circuits).