Gradient Program in HPLC

[saipraveen7]

Can anyone please provide me the links where I can get detailed information on different gradient curves (Ex. curve 4, curve 6, curve 9 etc.) that can be used in HPLC during a gradient program and their advantages

- P.Sai Praveen

[bisnettrj2]

Which brand/model of pump are you using?

[tom jupille]

and their advantages

Essentially there are no advantages. Anything you can do with a curved gradient you can do as well (with fewer transfer problems) using a series of linear segments. The curved gradient profiles are a relic from 40 years ago in the days before digital electronics (curved profiles are easier to generate with analog circuits).