Uncertainity measurements in chromatography

[bhuva31]

Dear All,

Could any one help me in listing of parameters to be done for uncertainity measurements for chromatograpghy like GC, GC-MS and LC-MS/MS.
Even relevant literaturs also will do.

Thanking you in advance.

With best regards

N.Bhuvaneswaran

[Hemal]

Uncertainity of chromatography is associated with method of analysis.

It can be evaluated from the method validation parameteres like Secificity, Precision, Accuracy & range.

More comments please.

Regards,
Hemal

[pawan ratra]

You can refer the following link

http://www.fda.gov/ora/science_ref/lm/v ... _04_06.pdf

[Poskrobko Jan]

EURACHEM/CITAC Guide
Quantifying Uncertainity in Analytical Measurement
Example 4 (phosphorous pesticide by GC)

You can download id from EURACHEM site

[vestelshirley]

for cal curves, there are formulas for calculating the uncertainty in the concentration based on the uncertainty in the slope and intercept.