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preparative LC system bleeding

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preparative LC system bleeding

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jiangds06
200ml of mobile phase was collected from 21mm column, some system bleeding(white solid, slightly soluble in mobile phase) was found. I just wonder if column bleeding produce these stuff?

Re: preparative LC system bleeding

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Gerhard Kratz
My guess is the following:
It is some buffer salt coming off from the column. Column was stored in mobile Phase for a while. Right?
What is your mobile Phase composition?
Gerhard Kratz, Kratz_Gerhard@web.de
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