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high pressure

Posted: Wed Aug 10, 2005 1:54 am
by moon704520
hi,everybody! I am using varian 320 prep,last time I find that the system pressure was too high,about 1600psi at 80ml/min ,but when I disconnect the lines between the column and the detector ,I found that the system pressure did decrease at 990psi,I do believe that somethong wrong with the detector , in fact ,I think that my detector was blocked ,if it is the trueth ,what can I do next?

Posted: Wed Aug 10, 2005 2:02 am
by Carlos Teixeira
Dear moon704520,

You must evaluate all probable points. Did you use buffers in you system?
What is the normal pressure of your system?

Have good elutions!!!

Carlos Teixeira