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high pressure

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

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hi,everybody! I am using varian 320 prep,last time I find that the system pressure was too high,about 1600psi at 80ml/min ,but when I disconnect the lines between the column and the detector ,I found that the system pressure did decrease at 990psi,I do believe that somethong wrong with the detector , in fact ,I think that my detector was blocked ,if it is the trueth ,what can I do next?

Dear moon704520,

You must evaluate all probable points. Did you use buffers in you system?
What is the normal pressure of your system?

Have good elutions!!!

Carlos Teixeira
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