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Erratic recoveries in manual injections
Posted: Mon Oct 28, 2013 6:25 pm
by wrb10dana
Can anyone suggest a reason for variations seen in manual injections of standard directly into a GC injection port? I am working with an Agilent 6890N GC/5973 Inert GC. Inj port temp = 180 C; split 30:1; 1 uL inj vol. There are no leaks anywhere in the system. Thanks.
Re: Erratic recoveries in manual injections
Posted: Tue Oct 29, 2013 8:12 am
by Peter Apps
Poor syringe handling is by far the most likely.
Peter
Re: Erratic recoveries in manual injections
Posted: Tue Oct 29, 2013 12:50 pm
by rb6banjo
What is the solvent for your samples?
Re: Erratic recoveries in manual injections
Posted: Tue Oct 29, 2013 7:08 pm
by James_Ball
Poor syringe handling is by far the most likely.
Peter
The time the needle is in the injection port can make a difference in your analyte response. Also the needle depth at time of injection or the speed you inject. It takes a lot of practice to be reproducible with manual injection techniques, but it can be done.